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Here we present non-enzymatic H 2 S production in vitro and in blood via a reaction specific for the SAA cysteine serving as substrate and requires coordinated catalysis by Vitamin B 6 , pyridoxal(phosphate), and iron under physiological conditions

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The gastrocnemius muscles were then excised, washed in cold phosphate-buffered saline, snap-frozen in liquid nitrogen-cooled isopentane, and stored at 80 C until analysis [2]

The ability to quantify the extent of glutathionylation would represent a substantial improvement in this approach, but would require double labelling using two different labels for the protein of interest and the BioGEE and as such poses significant technical challenges

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