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Confocal microscopy of reporter proteins transfected into cells has demonstrated that ROS are produced in localised places on the mitochondria, where electrons leak from the electron transfer chain (ETC) in brief pulses (107)

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However, the exhaustion-induced loss of function by CD8+ T cells serves to limit immunopathology in persistent infections, and the exuberant response of VHL-deficient T cells also mediates host death when mice are infected with a strain of virus that cannot be efficiently cleared by the immune system

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