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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

It is greater in normal cattle than in other normal domestic animals

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The use of FAs rather than carbohydrates when CoA levels are limited could potentially be driven by a lower K m for acyl-CoA synthetases compared with that for pyruvate dehydrogenase 60,61

doi: 10.4161/oxim.2.4.8876