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Description

Mice with hepcidin-resistant ferroportin accumulate iron in the retina

Growth hormone plays a fundamental role in preserving lean tissue during caloric deficit

Okamoto A, Iwamoto Y, Maru Y

2020;223(5):733.e1-14

The gel was crushed and the DNA was eluted twice from the crushed gel with 200 L of diffusion buffer (10 mM Tris, 25 mM sodium chloride, 1 mM EDTA, pH 8), at 70 C for 1 hour followed by 30 minutes, with mild shaking (1500 rpm)