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Subsequently, the UltraSYBR Green Mixture qPCR kit (Mei5 Biotechnology, Co., Ltd.) was used to perform RT-qPCR according to the manufacturer's protocol

There is evidence that all four subunits of GIRK channels are expressed at varying levels in the pancreas (Tanizawa et al., 1996), pancreatic islet cells (Yoshimoto et al., 1999), and insulinoma (Stoffel et al., 1995

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Regarding corticosterone levels, which directly indicate stress in murine models, a decrease was noted shortly after DSIP exposure

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