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An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow

[11] prokaryotes and protists, and in mitochondria and chloroplasts Iron Many bacteria contain a form of the enzyme with iron (Fe-SOD)

The same principle applies to many other naturally occurring antioxidants, which are found as mixtures of multiple isomers with slightly different 1-electron reduction potential (the ease with which they can accept a single electron)