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Intracellular IGF-1 is mainly expressed as proIGF-1Ea, not mature IGF-1 Using RT-PCR analyses, we previously demonstrated that skeletal muscles, adipose tissues and liver of several mammalian species mainly expressed the IGF-1Ea isoform, which represents about 90% of IGF-1 transcripts 5

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Thus, it was determined that 20 M leonurine, when supplemented to the in vitro culture medium, was the most effective dose for embryo development, as indicated by the blastocyst formation rates

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