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However, the combined treatment of IGF-1 and cGP (both at 100 nM) produced a similar growth rate to that of IGF-1 alone during the first 4 days of culture, then showed a significant inhibition at day 6 when compared to the treatment with IGF-1 alone (Fig

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The cells were cultured in RPMI1640 (FUJIFILM Wako Pure Chemical Corporation), supplemented with 10% heat-inactivated fetal calf serum (Capricorn Scientific), penicillin (100 U/ml) and streptomycin (100 g/ml) (FUJIFILM Wako Pure Chemical Corporation) and incubated at 37C in 95% humidified air with 5% CO2