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At the end of the treatment the cells were collected by trypsinization and counted using Countess Automated Cell Counter from Life Technologies (Gaithersburg, MD, USA) according to the manufacturers instruction

343 A dietary restriction regimen retarded the progression of neuropathological, behavioral, and metabolic abnormalities in an HD model, resulting in an extension of life span by increasing brain-derived neurotrophic factor and chaperone heat-shock protein-70 (HSP70) levels in the striatum and cortex, the mechanisms of which still need further explanation

and a brother, Henry Burton

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Cell 172, 409e21