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In research settings, NNMT inhibition is commonly used to explore how altering that branch point can shift the balance of methyl donor consumption, influence measurable pathway intermediates, and change downstream signatures that relate to energy handling, lipid pathways, and mitochondrial capacity depending on model and assay conditions

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Behavioral measurements should primarily include the percentage of time spent in the open quadrant, the percentage of entries into the open quadrant, the number of times the animal peeks down from the edge of the platform while in the open or closed quadrant, and the number of stretch-attend postures from the closed quadrant to the open quadrant

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Mengel, K., Krmer W, Isert B, Friedberg KD