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For cell scratch assay, HaCaT cells (13 1e6) or human keratinocyte primary cultures (5 1e5) were seeded in six/twelve-well plates with Dulbeccos modified Eagles medium (DMEM) containing 5% heat-inactivated FBS or with dermal cell basal medium supplemented with keratinocyte growth kit components (obtained from ATCC, USA)

We recommend blocking free thiols with alkylating agents like NEM or IAM during protein extraction to stabilize the modification

Accessed 11/11/2022, Simpson BW

Featuring Juvderm Voluma XC, an FDA-approved hyaluronic acid filler designed for deep structural support, contouring, and long-lasting volume restoration in the cheeks and chin

Its worth noting that the risk of side effects may vary from person to person, and some individuals may not experience any adverse reactions