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Broadhouse et al demonstrated a clear protection of the left hippocampus with 6 months of high-intensity RT (3 sets of 8 repetitions at 80% 1RM, two or three times per week) compared to shaman effect that persisted 1-year post-intervention in the 200 older adults studied (Broadhouse et al., 2020)

(1999) 277:L1138

After protein expression, E.coli cells are harvested by centrifugation, superatant is removed and cell pellet is resuspended in lysis buffer of choice