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Using the ratiometric redox sensitive GFP (roGFP) construct, we show that both moderate raise of ambient temperature (27-29C) and more intense high stress (37C) lead to oxidation of the roGFP both in the cytosol and the nuclear compartment, but with different kinetics depending on the heat treatment

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Matter 37 , 785789 (1988)

Sensing protein-protein interactions Intein biosensors for protein-protein interactions utilize PTS facilitated by split intein fragments that have low binding affinity for each other