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To analyse reduced and oxidized PDI by western blot, cells were lysed in 100 mM Tris-HCl pH 6.8, 2% SDS supplemented with PhosSTOP and cOmplete protease inhibitor cocktail and 40 mM ethylmaleimide to block free cysteines

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Some of the largest clinical trials, in fact, had to be aborted because the patients receiving antioxidants had a higher incidence of cancer than patients who did not receive them

Nrf2 is sensitive to maternal immunological status