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Additionally, the fluorescence intensity of cytosolically released Halo-Rac1 increased continuously after EV addition, reaching approximately six-fold the initial amount 12 h later (Supplementary Fig

coli induced with 0.5 mM IPTG and grown overnight at 20C at 150 rpm

A key feature of the inactive state is the presence of a major hydrogen bond network (MHN) involving residues N111 3.35 , N295 7.46 , D74 2.50 and N298 7.49 (superscripts denote BallesterosWeinstein numbering 96 ), which acts as a molecular switch that stabilizes the inactive conformation

Biochem J 312(Pt 3): 713716