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Description

After removing the final wash, the beads were incubated twice at room temperature in 80 l of the digestion buffer (2 M urea, 50 mM Tris-HCl, 1 mM DTT and 0.4 g of trypsin) while shaking at 1,000 rpm

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This demonstrates that DHEA caused a systemic inflammatory response and activated local ovarian signaling pathways in the ovaries

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Gao Q, Li C, Zhong P, Yu Y, Luo Z, Chen H

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