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To generate K63-linked I3C di-ubiquitin, 100 M Ub I3C was incubated with 800 nM mUbe1, 15 M Ubc4, 2.5 M Rsp5, and 5 mM ATP for 60 to 90 min at 30C in the ubiquitination buffer

Domain Weighting for Hospitals Receiving Scores on Fewer Than Four Domains In the FY 2015 IPPS/LTCH PPS final rule (79 FR 50084 through 50085), we adopted a policy that hospitals must receive domain scores on at least three of four quality domains in order to receive a TPS, for the FY 2017 program year and subsequent years

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