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Treatment of HepG2 cells with different concentrations of NFT or DEM for 24 h led to a concentration-dependent increase in Nrf2 and GCLC protein levels, whereas no significant change was observed in the levels of the modifier subunit, GCLM (Fig

Afterward, sections were washed three times with TBS and incubated with ImmPRESS-AP anti-rabbit IgG (alkaline phosphatase) polymer detection reagent (Vector Laboratories: MP-5402) for 30 min at room temperature