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separating the two applications by several hours is often advised

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This depends on the dose and frequency: 5mg vial: At 5mg 3x/week = less than 1 week (single dose per vial) At 5mg 2x/week = less than 1 week 10mg vial: At 5mg 3x/week = ~2.3 days (2 doses per vial) At 5mg 2x/week = 3.5 days (2 doses per vial) At 10mg 2x/week = 1 dose per vial MOTS-C is consumed faster than most peptides due to the higher per-dose amounts