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In practice, stable-isotope tracing metabolomics generally requires a prior knowledge of possible metabolite targets, leading to the ab initio elucidation of previously unknown metabolic reactions a great challenge 21,25

Biomolecules 14:593

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Apoptosis induction in gastric mucous cells in vitro : lesser potency of Helicobacter pylori than Escherichia coli lipopolysaccharide, but positive interaction with ibuprofen

In this way, the "liver filter" is skipped and the first-pass metabolism that usually occurs in the liver is avoided, which deactivates a large part of the Glutathione molecule, making it less functional and effective