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However, in Be(2)-C cells, it reduced ATP content by more than half ( Figure 3D ) without increasing cell death ( Figure 4A ), indicating a cytostatic rather than cytotoxic effect

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Coimmunoprecipitation (Co-IP) Cells were washed with PBS and lysed on ice for 30 min with IP lysis solution (Beyotime) supplemented with protease and phosphatase inhibitors (Thermo Fisher Scientific)

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