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The entire procedure typically takes only a few minutes, and multiple trigger points can be addressed in one session if needed

Non-specific antibody binding was reduced by incubating in 10% donkey serum (Sigma-Aldrich, UK) diluted in PBS for at least 30 min, followed by simultaneous incubation in primary antibodies (a melanocyte marker with a target antibody

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Semaglutide is a glucagon-like peptide-1 (GLP-1) analog that was initially approved in 2020 as a treatment method for improving glycemic control in patients with Type 2 diabetes