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The cells treated for 1 h with 200 M of the ROS inducer TBHP were used as a positive control

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Small pieces of rectal tissue were also collected and stored in paraffin for later analysis

Amino acid metabolism is a fundamental biological process that not only provides building blocks for protein synthesis, but also supports various cellular functions, including redox homeostasis, energy production, and epigenetic regulation [7,8,9]

They found that microbial -diversity decreased as the duration of illness increased, meaning that the variety of gut bacteria reduced over time in individuals with BD (Painold et al., 2019)