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We then evaluated the capacity of TF to protect against ferroptotic cell death, which is characterized by intracellular iron accumulation and an imbalance of the antioxidant system

By Day 15, alpha-fetoprotein (AFP), a hallmark of immature hepatocytes, demonstrated a notable increase, coupled with the emergence of early hepatic metabolic proteins like CYP2E1, signaling the onset of hepatic differentiation (Fig

10.3892/etm.2018.7014 104 LuanpitpongS.ChanvorachoteP.NimmannitU.LeonardS

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a , HT1080 OE GFP-GPX4 WT and HT1080 OE GFP-GPX4 R152H were treated with 4 M RSL3, 30 g/ml cycloheximide, and 100 M a-Tocopherol for 0, 2, 4, or 6 hours before Western Blot analysis of GPX4 and GADPH