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Indeed, antigen retrieval treatments in a citrate buffer with heat (boiling, steaming, microwave) are considered to denature SOD1 proteins, which could efficiently expose the epitope for misfolded-SOD1 antibodies [72] but appears not to describe the discrepancy on the immunohistochemical detection of misfolded SOD1 (Table 1)

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Extracellular matrix remodeling in vascular disease: defining its regulators and pathological influence