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UVvisible spectral studies of human CGL reacting with cysteine Spectra of CGL 100 g or 2.25 nmol human recombinant CGL (Cayman Chemicals, 95% pure) in a 400 L volume consisting of 100 mM potassium phosphate (pH 7.2) and either 0 or 100 M cysteine at 22 C were collected from 290 to 450 nm at a rate of 24,000 nm/min

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