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Description

Jakonen, Teppo 2019

Standard (IL-1 - 1 g/mL) and supernatant from the extract treated cells were added to the wells, followed by incubation for 2 h at room temperature

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For PttGSTF1, before crystallization, the protein ( ca 1 mL at 600 M) was treated with 10 mM GSH for 30 min, desalted on G25 columns and concentrated to the indicated concentration using Amicon Ultra centrifugal filters, Ultracel 10 K Membrane from Millipore