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Besides, berberine down-regulates the expression of acetylcholinesterase and inhibits acetylcholinesterase activity in Alzheimers disease model mice hippocampus which induced by heavy metals, in addition, berberine normalizes the production of inflammatory factors, such as tumor necrosis factor-alpha (TNF-), IL-6, IL-1 (Hussien et al., 2018)

Complementary DNA was synthesized using the RevertAid First Strand cDNA Synthesis Kit (K1622, Thermo Scientific, Vilnius, Lithuania)

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They found no such association between overall caffeine intake and increased risk of primary open-angle glaucoma