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Front Immunol (2019) 10:1587

Multiplexable, Locus-Specific Targeting of Long RNAs With CRISPR-Display

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Isolation of crypts, culture of intestinal organoids and treatments For murine organoid culture: ileum and colon tissues of mice were removed, wash with cold PBS, opened longitudinally, cut into pieces and then incubated on ice in buffer (1xPBS, 8 mM EDTA, 0.5 mM DTT) for 2045 min