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Aune D, Giovannucci E, Boffetta P, et al

Fibroblasts were cultured in DMEM/F12 (Life Technologies) supplemented with 20% FBS (Pan Biotech) and penicillin/streptomycin

Store the solid in a desiccated, light-protected container at low temperature with a tight seal

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Single cell mtDNA copy number calculation Using mgatk 20 , the mtDNA copy number at the single-cell level in mtscATAC-seq samples was calculated, computed as the (total bases of deduplicated reads mapped to the mtDNA genome) / (length of the mtDNA genome)