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The antigenantibody complex was bound to protein A/G magnetic beads for 1 h at 25 C, washed with IP Lysis/Wash buffer and purified water, eluted with the antigen/antibody complex, and analyzed using standard immunoblotting protocols

A 2022 study involving 56 people with rheumatoid arthritis found whole-body cryotherapy significantly reduced pain compared to control groups, with lower pain levels maintained at 12-week follow-up