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The content that mixes PK-2 is until evenly and through P-1 carrying out recirculation.Make temperature maintenance at 50-60 .Initial nitrogen NF stream is opened C-2 (vacuum pump) subsequently.It should be noted that and preferably avoid the product foam that rises.Subsequently batch is cooled to 45-50 , opens vacuum and nitrogen NF simultaneously.When product is cooled to said temperature, close C-2, remove any vacuum with nitrogen NF.Nitrogen NF stream stays open.Purify dump valve with product subsequently, collect sample or packing product then.Product is transferred to preweighted clean HDPE top to be closed in the container.Close A-2, P-1 and nitrogen NF, accomplish in batches, and on the TIC-2 drawing, indicate

In addition, glutamine uptake can be negatively regulated by retinoblastoma (Rb) tumor suppressor family, whose deletion increases glutamine uptake via the E2F -dependent upregulation of SLC1A5/ASCT2 and GLS1 [22]