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IGF-1 treatment of differentiated Th17 cells did not increase the percentage of IL-17 positive cells but did increase IL-17 production as measured by mean fluorescent intensity flow cytometrically (Fig

Jas et al., 2015)

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Membranes were blocked with 5% milk in 0.1M TBST and probed with primary antibody: GAPDH (Mouse, Abcam G8795,1:1000) was used as an internal control for the concentration of protein loaded, while anti-MBP (Rat, BioRad MCA4095,1:1000) was used to detect MBP