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For the metabolomic analysis, 100 L of serum were mixed with 400 L of extraction solvent (methanol:acetonitrile:acetone = 1:1:1, v/v)

It is considered to represent a breakdown of cell membranes

The A549 cells were incubated with two heavy metal toxins for 24 and 48 h at two dose levels: 50 and 100 mol/L CdCl and 100 and 200 mol/L cisPt, alongside two control samples without the addition of the toxins

Clearly, it is well known that many individuals with vitamin B 12 deficiency are clinically asymptomatic and therefore the overall rate of vitamin B 12 deficiency identified in our analyses may be an underestimate

It is essential to diagnose the condition upon noticing the related symptoms