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RNA was extracted from the collected cells and lysed into fragments utilizing ultrasound, and protein A/G magnetic beads were prewashed and immunoprecipitated with anti-m 6 A antibody (5 l, Activemotif, 91261), taking the Input group as the control [38]

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For digestion, the protein solution was reduced with 5 mM dithiothreitol for 30 min at 56 C and alkylated with 11 mM iodoacetamide for 15 min at room temperature in darkness

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