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Causes of vitamin B12 deficiency include food cobalamin malabsorption (low intestinal absorption of the vitamin from food) due to intrinsic factor receptor deficiency (a substance needed for absorption of B12), impaired calcium availability, gastrointestinal disorders such as chronic gastritis, Crohns disease, and other bowel inflammatory disorders, surgery on the digestive tract such as total or partial gastrectomy, or autoimmune conditions

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500 ng of RNA was converted to cDNA using the High Capacity Reverse Transcription kit (Life Technologies) according to the manufacturers instructions