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This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]

We have previously demonstrated that these fluxes of cytosolic glutamate can occur without significant increases in oxidative stress or altered cell viability (16)

Dual roles for HMGB1: DNA binding and cytokine

Selenium rescues orbital fibroblasts from cell death induced by hydrogen peroxide: Another molecular basis for the effects of selenium in graves orbitopathy

This process generates inflammation and oxidative stress