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Materials and Methods Animals A total of one hundred adult male Swiss albino mice ( n = 100

Skeletal muscle membrane preparation Wild-type RyR1 was purified from rabbit skeletal muscle (purchased from Pel-Freeze Biologicals), and R615C RyR1 was purified from muscle from pigs homozygous for this mutant (purchased from Boyle Farms, Moorehead, Iowa) as described before 38

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Safe for daily twice-daily use as directed