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Acquired data were processed with Bruker TopSpin and analyzed using NMRFAM-SPARKY software

A potential direct metabolic impact of SUN1 H118Y was tested in human hepatoma (Huh7 and HepG2) cells using semi-quantitative lipid staining (BODIPY 493/593) and determination of insulin-stimulated AKT phosphorylation via immunoblot, as well as transcriptional analysis (qPCR) of lipid-related gene expression

Risk of bias and quality of evidence The authors used the Risk of Bias (RoB 2)19,20 tool to assess the risk of bias for other fundamental elements, expressed as very serious, serious, or non-serious