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A least 2 weeks prior to tissue collection, a cohort of animals were maintained in a reverse light-dark cycle in which lights were turned off at 6am and lights were turned on at 6pm

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For example, when first starting, you might only perform lower-intensity double leg hops in place for 10-15 seconds and or double leg sub max vertical jumps for 3-5 repetitions, 1-2 days a week