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Total RNA was isolated using Qiagen RNeasy mini kit (Cat # 74106), RNA sequencing libraries were prepared with Illumina-compatible NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, MA, USA) were carried out by Genotypic Pvt.Ltd

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Results: Before treatment, patients with a positive prognosis typically showed elevated frequencies of T cells, CD4+ T cells, classical monocytes, CD8+ T cells, and non-classical monocytes in their PBMCs compared to those with a less favorable prognosis