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Store the vial in controlled room temperature, avoiding direct light

The initial metabolic shifts in microglia tend to trigger neuroinflammation and phagocytic suppression [65, 76]

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We calculated the mean of non-displaceable binding potential as a marker of microglial activation, and the mean influx rate as a marker of BBB leakage in these three shells and the whole white matter

Following staining, slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 min