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Acknowledgments We thank staff members and postgraduate students from the Guangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation for providing technical assistance

10.1210/endo-124-6-3050 80

10.1016/0006-8993(78)90899-5 Brain Res

For beginners, starting with a 1% concentration is recommended

In canonical Wnt/-catenin signaling, the secreted Wnt ligands bind with the Frizzled receptor, which further inactivates glycogen synthase kinase-3 (GSK-3), which is an enzyme responsible for the ubiquitination-mediated degradation of -catenin 4,5