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A Superdex 200 Increase 10/300 GL column was calibrated with apoferritin (440 kDa), alcohol dehydrogenase (150 kDa), conalbumin (75 kDa), ovalbumin (44 kDa), carbonic anhydrase (29 kDa), and ribonuclease (13.7 kDa), which were purchased from GE Healthcare