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On the day of the assay, cells were lifted, washed with 1X PBS, and stained (1:1000) with eBioscience Fixable Viability Dye eFluor 780 for 30 minutes on ice

The expression of Slc7a11 in the kidneys increased after IRI, reaching a peak at days 30 and 45, when mature TLS are formed (Figure 3A)

Figure 4 represents the factors affecting the optical properties of CDs

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