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doi: 10.1136/jitc-2020-001223 10 KumarVPatelSTcyganovEGabrilovichDI

D.ChuecaA.SahrawyM

Nat Commun 2016;7:11971

DNA vectors Expression vector for AHCY was obtained by amplifying the mouse AHCY ORF from the HA-AHCY vector 59 using the primers 5 -ATAGTCGACGCCACCATGTACCCATACGATGTT-3 and 5 -TATGCTAGCTTCAGTAGCGGTAGTGATCAGGCT-3 , then ligating the SalI/NheI-digested PCR product into the SalI/NheI-digested pSELECT-HYGRO-MCS vector (Invivogen) using Ligation high Ver.2 (Toyobo)