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6 Immobilization of nitrilase for nicotinic acid production The immobilization (Figure 5) of nitrilase has also been performed by various researchers to increase the stability and reusability of enzymes on an industrial scale ( Escherichia coli was immobilized using an alginate (2.5%) matrix, and immobilized cells were reused for up to 25 cycles with 100% activity

This well-established importance underlies the role of vitamin B12 deficiency in mood imbalances, psychotic disorders, and neuronal health, particularly in elderly patients, where this deficiency is estimated to affect up to 40% of the population (72)

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