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Progress of the reaction was monitored by analytical HPLC using C4 analytical column and a gradient of 060% buffer B (Acetonitrile mixed with 0.1% TFA) over 30 min

10.2165/00002018-200326140-00002 137 PachmanD

Like CDS, protein sequence and molecular weight SlGST shows a wide variation in their isoelectric point (pI) ranging from 4.67 (SlGSTU4) to 9.43 (SlEF1B2) where 77 are acidic and 13 are basic

The hydrogen and carbon atom positions in the 1 H-NMR and 13 C-NMR spectra were supported by the dept-135 or COSY experiments and by 2D-NMR map analysis of the Heteronuclear Multiple-Quantum Correlation (HMQC) and Heteronuclear Multiple Bond Correlation (HMBC), Nuclear Overhauser Enhancement Spectroscopy (NOESY) if measured

& Mukherjee, P