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Laboratory Baseline hemoglobin electrophoresis (HPLC) performed using D-10 (Bio-Rad, Marnes La Coquette, France) for initial Hb F levels, CBC with reticulocyte count using Sysmex XT-1800i (Sysmex, Kobe, Japan), markers of hemolysis in form of total serum and indirect bilirubin, alanine aminotransferase (ALT), aspartate transaminase (AST ) , and serum creatinine using Cobas Integra 800 (Roche Diagnostics, Mannheim, Germany) were performed before starting treatment with l-glutamine and on monthly bases through the 24 weeks, while serum ferritin level was measured at entry of the study on Immulite 1000 analyzer (Siemens Healthcare Diagnostics, Marburg, Germany)

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Patients frequently pair it with TB-500 for systemic healing support