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Attached THP-1 cells were stimulated with 100 ng ml 1 lipopolysaccharide (Sigma) for 3 h and treated with DMSO, GSTO1 inhibitors or Glyburide (Enzo Life sciences) in serum-free media for 1.5 h followed by addition of 5 mM ATP (Sigma) for 30 min

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GPX4 plays a pivotal role in regulating lipid peroxides within cells and the reduction in GSH and GPX4 that we demonstrate in the IB3-1 AECs following FAC and erastin treatment is likely responsible for the increased lipid peroxidation that we found, as evidenced by the increase in MDA and C11-BODIPY

Zhu et al., 2023

Quick example: You usually crash at 3 p.m., but after one drip you sail through the afternoon without reaching for extra coffee